PathHunter® CHO-K1 CNR1 Bioassay Kit

The PathHunter CHO-K1 CNR1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Melanin Concentrating Hormone (6-17)) based on activation of CNR1 GPCR activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged CNR1 and EA-tagged β-Arrestin-2. Activation of CNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0959Y2-00120 1000 dp (10 x 96-well) $7,729.00
93-0959Y2-00119 200 dp (2 x 96-well) $2,249.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Cannabinoid receptor 1
Group
Cannabinoid
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
AY225225
Agonist/Ligand
CP 55,940
Assay Measures
β-Arrestin
Bioassay Target Name
CNR1
Assay Qualified With
CP 55,940
This representative graph is an example of a typical dose-response curve for the CNR1 Bioassay generated using the protocol outlined in this user manual. The data shows potent, dose-dependent recruitment of β-arrestin, when treated with CP 55,940.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gi/Go