The PathHunter CHO-K1 C5AR1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. TNFα (human)) based on activation of C5AR1 GPCR activity via detection of IkB degradation. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged . Activation of C5AR1 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.