PathHunter® CHO-K1 AGTR1 Bioassay Kit

The PathHunter CHO-K1 AGTR1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Angiotensin II) based on activation of AGTR1 GPCR activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTR1 and EA-tagged β-Arrestin-2. Activation of AGTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0312Y2-00126 1000 dp (10 x 96-well) $7,729.00
93-0312Y2-00125 200 dp (2 x 96-well) $2,249.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Angiotensin II receptor, type 1
Group
Angiotensin
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
NM_000685
Agonist/Ligand
Angiotensin II
Assay Measures
β-Arrestin
Bioassay Target Name
AGTR1
Assay Qualified With
Angiotensin II
The graph is an example of a typical dose response curve for the AGTR1 Bioassay generated using the protocol outlined in the User Manual. The data shows potent, dose-dependent recruitment of β-arrestin, when treated with Angiotensin-II.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gq