PathHunter® eXpress C5AR1 Activated GPCR Internalization Assay

PathHunter® eXpress Activated GPCR Internalization cells are engineered to co-express an untagged GPCR, Enzyme Acceptor (EA) tagged β-Arrestin, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR induces β-Arrestin recruitment, followed by internalization of the Receptor/Arrestin-EA complex in PK-tagged endosomes. This forces complementation of the two β-galactosidase enzyme fragments (EA and PK), forming functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0777E3CP5M 200 dp (2 x 96-well) $2,279.00
93-0777E3CP5L 1000 dp (10 x 96-well) $7,479.00

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Target/Description
Complement component 5a receptor 1
Group
Complement peptide
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_001736
Agonist/Ligand
Recombinant Human Complement C5a
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gi/Go
  • [User Manual] PathHunter® eXpress Activated GPCR Internalization Assay

    PathHunter® eXpress Activated GPCR Internalization Assays are ready to use complete kits that contain everything you need to measure arrestin bound internalized GPCRs in live cells without the hassle of using antibodies, radioactivity or elaborate imaging. The eXpress kits include single use vials of frozen cells stably expressing the GPCR of int...

    [User Manual] PathHunter® eXpress Activated GPCR Internalization Assay