PathHunter® BDKRB2 Bioassay Kit

The PathHunter CHO-K1 BDKRB2 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Bradykinin) based on activation of BDKRB2 GPCR activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0189Y2-00114 1000 dp (10 x 96-well) $7,729.00
93-0189Y2-00113 200 dp (2 x 96-well) $2,249.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Bradykinin receptor B2
Group
Bradykinin
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
NM_000623.3
Agonist/Ligand
Bradykinin
Assay Measures
β-Arrestin
The following graph is an example of a typical dose-response curve for the BDKRB2 Bioassay, generated using the protocol outlined in the user manual.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gq