InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay

InCELL Hunter assay detects degradation of a partner protein that is ordinarily stabilized by its natural interaction with the target protein. The cell lines are engineered to co-express an untagged target protein, and an Enzyme Donor (ED) tagged partner protein. Either protein may be full length or truncated. Addition of inhibitors disrupting the interaction of the two proteins results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
96-0009E3CP16M 200 dp (2 x 96-well) $1,479.00
96-0009E3CP16L 1000 dp (10 x 96-well) $4,977.00

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BCL2-like 1
Cell Background
Receptor Species
Accession Number
Assay Measures
Intracellular Binding
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, signal was detected using the InCELL Hunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
See User Manual for Product Configuration Details
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
  • [User Manual] InCELL Hunter™ eXpress Assays

    InCELL Hunter™ eXpress Assays provide a cell-based approach to evaluate changes in protein stability upon compound binding. These assay kits have been validated for use in 96-well and 384-well microplate formats. The eXpress kits contain all the materials needed for a complete assay including cells, cell plating reagents, detection reagents, and plates. The pre-validated, cryopreserved eX...

    [User Manual] InCELL Hunter™ eXpress Assays