Find a Product

PathHunter® Aflibercept Bioassay Kit

The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0996Y1-00006 1000 dp (10 x 96-well) $8251.00
93-0996Y1-00005 200 dp (2 x 96-well) $2352.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Kinase Insert Domain Receptor
Group
Receptor Tyrosine Kinase
Cell Background
HEK 293
Species
Human
Accession Number
NM_002253.2
Agonist/Ligand
VEGF-165
Assay Measures
Dimerization
Bioassay Target Name
KDR/KDR
Assay Qualified With
Eylea®
Drug Trademark Owner
Eylea® is a registered trademark of Regeneron Pharmaceuticals, Inc.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
  • [User Manual] PathHunter® Aflibercept Bioassay Kit

    PathHunter® Aflibercept Bioassay kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study aflibercept potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay plat...

    [User Manual] PathHunter® Aflibercept Bioassay Kit