PathHunter® Adalimumab Bioassay Kit

The PathHunter Adalimumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Recombinant Human BDNF) based on activation of IkB Pathway activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged IkB and EA-tagged . Activation of IkB stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc.. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0538B15-00132 1000 dp (10 x 96-well) $8,259.00
93-0538B15-00131 200 dp (2 x 96-well) $2,399.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Group
Pathway
Cell Background
A549
Receptor Species
Human
Accession Number
NM_020529
Agonist/Ligand
TNFα (human)
Assay Measures
IkB degradation
Bioassay Target Name
IkB
Assay Qualified With
Humira
Drug Trademark Owner
Humira is a registered trademark of AbbVie, Inc.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A