The PathHunter Adalimumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay is designed to measure IkB degradation as a result of TNF alpha-mediated activation of the NF-kB signaling pathway. When bound to ligand-neutralizing agents such as Adalimumab, TNFa is unavailable to stimulate its cognate receptor. This prevents the activation of the NF-ƙB signaling pathway and consequently, TNFa-mediated IkB degradation. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. Humira is a registered trademark of AbbVie, Inc.