PathHunter® Adalimumab Bioassay Kit

The PathHunter Adalimumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay is designed to measure IkB degradation as a result of TNF alpha-mediated activation of the NF-kB signaling pathway. When bound to ligand-neutralizing agents such as Adalimumab, TNFa is unavailable to stimulate its cognate receptor. This prevents the activation of the NF-ƙB signaling pathway and consequently, TNFa-mediated IkB degradation. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. Humira is a registered trademark of AbbVie, Inc.

Catalog No. Size Price Quantity
93-0538B15-00132 1000 dp (10 x 96-well) $9,980.00
93-0538B15-00131 200 dp (2 x 96-well) $2,979.00

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nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Cell Background
Receptor Species
Accession Number
TNFα (human)
Assay Measures
IkB degradation
Bioassay Target Name
Assay Qualified With
Drug Trademark Owner
Humira is a registered trademark of AbbVie, Inc.
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.