The PathHunter Adalimumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Recombinant Human BDNF) based on activation of IkB Pathway activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged IkB and EA-tagged . Activation of IkB stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc.. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.