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InCELL Pulse™ ABL1 Target Engagement Assay

InCELL Pulse cellular target engagement assays measure compound binding to a cellular target by detecting changes in protein thermal stability. In this assay, the target is fused to the enhanced ProLabel® (ePL) enzyme fragment and expressed in the selected cell background. The fusion is detected with high sensitivity through the addition of a substrate and complementary enzyme acceptor fragment (EA) in the presence of a cell lysis buffer. The ePL tag is an ideal fusion partner for InCELL Pulse for the following reasons: 1) ePL is a small unstructured peptide (42 aa) that does not affect the tagged protein’s thermal denaturation profile; 2) ePL enables simple, sensitive, and precise fusion protein detection; and 3) the sensitivity of ePL detection depends on the macromolecular state of the fusion protein. Whereas native properly folded ePL fusion proteins are detected with high sensitivity, denatured aggregated states of fusion proteins are poorly detected. The ability of ePL to report on a fusion protein’s macromolecular state is the basis of the InCELL Pulse technology because ligand binding can protect proteins from thermal denaturation. For a detailed description of the InCELL Pulse assay principle, please refer to the user manual. The provided cells are for single use only and have been prepared such that target expression reduces dramatically upon passaging.

Catalog No. Size Price Quantity
94-4001E1-01 200 dp (2 x 96-well) $819.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
ABL proto-oncogene 1, non-receptor tyrosine kinase
Group
Non-receptor tyrosine kinase
Cell Background
HEK 293
Species
Human
Accession Number
NP_005148.2
Agonist/Ligand
Dasatinib (BMS-354825)
Assay Measures
Intracellular Binding
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] InCELL Pulse™ Cellular Target Engagement Assays

    InCELL Pulse Cellular Target Engagement Assays are 96-well plate format kits for the assessment of inhibitor cellular potency. The assay uses DiscoverX’s proprietary enzyme fragment complementation (EFC) technology to measure compound-dependent thermal stabilization of target proteins in a cellular context. InCELL Pulse enables the measurement of quantita...

    [User Manual] InCELL Pulse™ Cellular Target Engagement Assays