SPRINTer™ Human BRD4 Protein Turnover Biosensor Cell-Based Assay Kit (K562)

Background Information:

The SPRINTer™ Human BRD4 Targeted Protein Degradation Cell-Based Assay Kit (K562) utilizes the SPRINTer™ K562 BRD4 Protein Turnover Biosensor Cell Line to monitor endogenous BRD4 protein levels and allow measurement of drug-induced targeted protein degradation (TPD). Genome engineering or editing techniques, such as CRISPR/Cas9, are used to introduce a small tag into the endogenous locus of interest. This allows for the quantitation of changes in BRD4 levels when cells are treated with therapeutic agents such as targeted protein degraders. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Epigenetic

Family:

Bromodomain-Containing

Sub Family:

Reader

Protein Name:

BRD4

Protein Aliases:

chromosome-associated protein

Accession Number:

NM_058243.2

UniProt Number:

O60885

Gene Name:

BRD4

Gene ID:

23476

Gene Aliases:

HUNKI|MCAP|CAP|HUNK1

Target Species:

Human

Cell Background:

K562