SPRINTer™ Human IKZF1 Protein Turnover Biosensor Cell-Based Assay Kit (K562)

Background Information:

The SPRINTer™ Human IKZF1 Targeted Protein Degradation Cell-Based Assay Kit (K562) utilizes the SPRINTer™ IKZF1 Protein Turnover Biosensor Cell Line (K562) to monitor endogenous IKZF1 protein levels and allow measurement of drug-induced targeted protein degradation (TPD). Genome engineering or editing techniques, such as CRISPR/Cas9, are used to introduce a small tag into the endogenous locus of interest. This allows for the quantitation of changes in IKZF1 levels when cells are treated with therapeutic agents such as targeted protein degraders. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Nuclear Protein

Family:

Transcription factor

Sub Family:

Zinc Finger

Protein Name:

Ikaros

Protein Aliases:

DNA-binding protein Ikaros | Ikaros family zinc finger protein 1|Lymphoid transcription factor LyF-1|protein phosphatase 1, regulatory subunit 92

Accession Number:

Q13422

UniProt Number:

Q13422

Gene Name:

IKZF1

Gene ID:

10320

Gene Aliases:

IK1|IKAROS|LYF1|ZNFN1A1|hIk-1|LyF-1|Hs.54452|PPP1R92

Target Species:

Human

Cell Background:

K562