PathHunter® Human VISTA/VISTA Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human VISTA/VISTA Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS VISTA/VISTA Dimerization Cell Line. This assay enables the assessment of anti-VISTA antibody-induced dimerization of VISTA/VISTA. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing VISTA fused to PK and another construct containing VISTA fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Checkpoint Receptor

Family:

T Cell Receptor

Protein Name:

VISTA

Protein Aliases:

Stress-induced secreted protein-1|Sisp-1|V-set domain-containing immunoregulatory receptor|V-set immunoregulatory receptor

Accession Number:

NM_022153.1

UniProt Number:

Q9H7M9

Gene Name:

VSIR

Gene ID:

64115

Gene Aliases:

C10orf54|SISP1|VISTA|PP2135|UNQ730/PRO1412

Target Species:

Human

Cell Background:

U2OS