PathHunter® Human SMO (W535L) MEM-EA Stable Cell Line Pharmacotrafficking Assay (U2OS)

Background Information:

The PathHunter Human SMO (W535L) MEM-EA Stable Cell Line Pharmacotrafficking Assay (U2OS) utilizes the SMO(W535L) MEM-EA Pharmacotrafficking cell line. The stable engineered cell line is designed for use in studying pharmacochaperones targeting SMO. This cell line enables assessment of chaperone-based stabilization of SMO(W535L) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant SMO(W535L), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant SMO, it is trafficked to the membrane, producing EFC signal. The Cell Line Assay also includes the cell line specific detection reagent required for optimal assay performance that is required as part of the label license in the purchase and use of the cell line.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Sub Family:

Frizzled

Coupling:

Gi/Go

Protein Name:

SMO

Protein Aliases:

Protein Gx

Accession Number:

NM_005631.4

UniProt Number:

Q99835

Gene Name:

SMO

Gene ID:

6608

Gene Aliases:

SMOH

Target Species:

Human

Cell Background:

U2OS