PathHunter® U2OS Siglec9 Internalization Cell Line

Background Information:

PathHunter® U2OS Siglec9 Internalization Cell Line is engineered to co-express the ProLink™ (PK) tagged Siglec9 receptor, and an Enzyme Acceptor (EA) tag localized to the endosomes. Ligand (e.g. Sialic Acid, anti-siglec9 antibody) binding to the receptor stimulates receptor internalization, resulting in colocalization of the receptor-PK with the EA in the endosomes, and forcing complementation of the two β galactosidase (β-gal) enzyme fragments (EA and PK). The resulting functional β-gal enzyme hydrolyzes substrate to generate a chemiluminescent signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Checkpoint Receptor

Family:

T Cell Receptor

Protein Name:

Siglec-9

Protein Aliases:

CDw329|Protein FOAP-9|CD329

Accession Number:

NM_014441.2

UniProt Number:

Q9Y336

Gene Name:

SIGLEC9

Gene ID:

27180

Gene Aliases:

CD329|CDw329|FOAP-9|siglec-9|OBBP-LIKE

Target Species:

Human

Cell Background:

U2OS