PathHunter® U2OS RANK NF-κB Signaling Pathway Reporter Cell Line

The PathHunter® U2OS RANK NF-κB Signaling Pathway Reporter Cell Line overexpresses RANK and measures NF-kB pathway activation through detection of induced expression levels of a tagged reporter protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1156C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS RANK NF-κB Signaling Pathway Reporter Cell Line is a stable, engineered cell line that overexpresses RANK. It can be used for studying drug candidates (small molecules or biologics) affecting the NF-kB pathway. This cell line enables assessment of NF-kB pathway stimulation based on changes in the expression of a NF-Kb response element-driven reporter protein. This product uses EFC technology. The included cell line expresses PL-tagged reporter protein under the control of a NF-Kb pathway-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of EFC signal upon addition of EA-containing detection reagent.  This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Signaling Pathway
Family:
Tumor Necrosis Factor Receptor Superfamily
Accession Number:
NM_003839.3
Target Name:
RANK-NF-kB
Target Aliases:
Tumor necrosis factor receptor superfamily, member 11a
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Signaling Reporters
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Signaling
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-406 PH Signaling Pathway Reporter Assay User Manual REV0

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Datasheets

93-1156C3

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