PathHunter® U2OS RANK NF-κB Signaling Pathway Reporter Cell Line

The PathHunter® U2OS RANK NF-κB Signaling Pathway Reporter Cell Line overexpresses RANK and measures NF-kB pathway activation through detection of induced expression levels of a tagged reporter protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1156C3 Size: 2 vials

Biological Information
Background Information:	The PathHunter® U2OS RANK NF-κB Signaling Pathway Reporter Cell Line is a stable, engineered cell line that overexpresses RANK. It can be used for studying drug candidates (small molecules or biologics) affecting the NF-kB pathway. This cell line enables assessment of NF-kB pathway stimulation based on changes in the expression of a NF-Kb response element-driven reporter protein. This product uses EFC technology. The included cell line expresses PL-tagged reporter protein under the control of a NF-Kb pathway-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of EFC signal upon addition of EA-containing detection reagent. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Signaling Pathway
Family:	Tumor Necrosis Factor Receptor Superfamily
Accession Number:	NM_003839.3
Target Name:	RANK-NF-kB
Target Aliases:	Tumor necrosis factor receptor superfamilymember 11a
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Signaling Reporters
Storage Conditions:	Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Signaling
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®