Biological Information

Background Information:

The PathHunter® U2OS PDGFRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PDGFRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

PDGFRalpha

Protein Aliases:

PDGFR-alpha|Alpha platelet-derived growth factor receptor|Alpha-type platelet-derived growth factor receptor|CD140 antigen-like family member A|CD140a antigen|Platelet-derived growth factor alpha receptor|Platelet-derived growth factor receptor 2|PDGFR-2|CD antigen CD140a

Accession Number:

NM_006206.4

UniProt Number:

P16234

Gene Name:

PDGFRA

Gene ID:

5156

Gene Aliases:

CD140a|PDGFR2|GAS9|RHEPDGFRA

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Functional Activity

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

PathHunter®