Biological Information

Background Information:

The PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting mRHO. This cell line enables assessment of chaperone-based stabilization of mRHO(P23H) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant mRHO(P23H), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant mRHO, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Opsin

Sub Family:

Orphan and other 7TM

Coupling:

Gt

Protein Name:

OPSD

Accession Number:

NM_145383.1

UniProt Number:

P15409

Gene Name:

RHO

Gene ID:

212541

Target Species:

Mouse

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Pharmacotrafficking

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Pharmacotrafficking

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®