PathHunter® Human MC4R(T162I) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human MC4R(T162I) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS) utilizes the U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking cell line. The stable engineered cell line is designed for use in studying pharmacochaperones targeting MC4R. This cell line enables assessment of chaperone-based stabilization of MC4R(T162I) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant MC4R(T162I), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant MC4R, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

MC4

Accession Number:

NM_005912.2

UniProt Number:

P32245

Gene Name:

MC4R

Gene ID:

4160

Target Species:

Human

Cell Background:

U2OS