PathHunter® Human IL-27 (IL27RA/IL6ST) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-27 (IL27RA/IL6ST) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IL27RA/IL6ST Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-27)-induced dimerization of IL27RA/IL6ST pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IL27RA fused to PK and IL6ST fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

IL-6 Receptor

Protein Name:

IL27RA

Protein Aliases:

IL-27R subunit alpha|IL-27R-alpha|IL-27RA|Cytokine receptor WSX-1|Cytokine receptor-like 1|Type I T-cell cytokine receptor|TCCR|ZcytoR1

Accession Number:

NM_004843.3

UniProt Number:

Q6UWB1

Gene Name:

IL27RA

Gene ID:

9466

Gene Aliases:

CRL1|TCCR|WSX1|UNQ296/PRO336

Target Species:

Human

Cell Background:

U2OS