PathHunter® Human IL-18 (IL18R1/IL18RAP) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-18 (IL18R1/IL18RAP) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IL18R1/IL18RAP Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-18)-induced dimerization of IL18R1/IL18RAP pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IL18R1 fused to PK and IL18RAP fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

IL-1 Receptor

Protein Name:

IL18R1

Protein Aliases:

IL-18R1|CD218 antigen-like family member A|CDw218a|IL1 receptor-related protein|IL-1Rrp|IL1R-rp|Interleukin-18 receptor alpha|IL-18R-alpha|IL-18Ralpha|CD antigen CD218a

Accession Number:

NM_003855.3

UniProt Number:

Q13478

Gene Name:

IL18R1

Gene ID:

8809

Gene Aliases:

IL1RRP

Target Species:

Human

Cell Background:

U2OS