PathHunter® U2OS GHR-JAK2 Functional Assay

Background Information:

The PathHunter® GHR-JAK2 Functional assay cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting CTKs. This cell line enables assessment of GHR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GHR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Cytokine Receptor

Family:

Prolactin Receptor

Protein Name:

GHR

Protein Aliases:

Somatotropin receptor [Cleaved into: Growth hormone-binding protein|GH-binding protein|GHBP|Serum-binding protein]

Accession Number:

NM-000163

UniProt Number:

P10912

Gene Name:

GHR

Gene ID:

2690

Gene Aliases:

GHBP

Target Species:

Human

Cell Background:

U2OS