PathHunter® Human Heregulin beta 2 (ErbB4/ErbB4) Stable Cell Line Dimerization Assay (U2OS)

Background Information:

The PathHunter Human Heregulin beta 2 (ErbB4/ErbB4) Stable Cell Line Dimerization Assay (U2OS) utilizes the ErbB4/ErbB4 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. heregulin β2)-induced dimerization of ErbB4/ErbB4. The included cell line employs the PathHunter Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing ErbB4 fused to PK and another construct containing ErbB4 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10 plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

HER4

Protein Aliases:

human epidermal growth factor receptor 4

Accession Number:

NM_005235.2

UniProt Number:

Q15303

Gene Name:

ERBB4

Gene ID:

2066

Gene Aliases:

ALS19|HER4

Target Species:

Human

Cell Background:

U2OS