PathHunter® Human Heregulin beta 2 (ErbB4/ErbB4) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human Heregulin beta 2 (ErbB4/ErbB4) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS ErbB4/ErbB4 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. heregulin β2)-induced dimerization of ErbB4/ErbB4. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing ErbB4 fused to PK and another construct containing ErbB4 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

HER4

Protein Aliases:

human epidermal growth factor receptor 4

Accession Number:

NM_005235.2

UniProt Number:

Q15303

Gene Name:

ERBB4

Gene ID:

2066

Gene Aliases:

ALS19|HER4

Target Species:

Human

Cell Background:

U2OS