PathHunter® U2OS CSF3R-JAK1 Functional Assay

Background Information:

The PathHunter® CSF3R-JAK1 Functional assay cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting CTKs. This cell line enables assessment of CSF3R activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CSF3R cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Cytokine Receptor

Family:

Prolactin Receptor

Protein Name:

CSF3R

Protein Aliases:

G-CSF-R|CD antigen CD114

Accession Number:

NM_000760

UniProt Number:

Q99062

Gene Name:

CSF3R

Gene ID:

1441

Gene Aliases:

GCSFR

Target Species:

Human

Cell Background:

U2OS