PathHunter® Human AVPR1A β-Arrestin Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human AVPR1A β-Arrestin Cell-Based Assay Kit (U2OS) utilizes the Human AVPR1A β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged AVPR1A target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

V1A

Protein Aliases:

AVPR V1a|Antidiuretic hormone receptor 1a|Vascular/hepatic-type arginine vasopressin receptor

Accession Number:

NM_000706.3

UniProt Number:

P37288

Gene Name:

AVPR1A

Gene ID:

552

Gene Aliases:

AVPR1

Target Species:

Human

Cell Background:

U2OS