PathHunter® Human BMP-7 (ACVR2/ACVR1) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human BMP-7 (ACVR2/ACVR1) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS ACVR2/ACVR1 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. BMP-7)-induced dimerization of ACVR2/ACVR1 pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress ACVR2 fused to PK and ACVR1 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RSTK

Sub Family:

Protein Ser/Thr

Protein Name:

ALK2

Protein Aliases:

Activin receptor type I|ACTR-I|Activin receptor-like kinase 2|ALK-2|Serine/threonine-protein kinase receptor R1|SKR1|TGF-B superfamily receptor type I|TSR-I

Accession Number:

NM_001616

UniProt Number:

Q04771

Gene Name:

ACVR1

Gene ID:

90

Gene Aliases:

SKR1|ALK2|ACVR1A

Target Species:

Human

Cell Background:

U2OS