PathHunter® Jurkat SIRPa Signaling Cell Line

Background Information:

The PathHunter® SIRPa Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD47) based activation of SIRPa receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged SIRPa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Inhibitory Receptor

Protein Name:

SIRPA

Protein Aliases:

SHPS-1|Brain Ig-like molecule with tyrosine-based activation motifs|Bit|CD172 antigen-like family member A|Inhibitory receptor SHPS-1|Macrophage fusion receptor|MyD-1 antigen|Signal-regulatory protein alpha-1|Sirp-alpha-1|Signal-regulatory protein alpha-2|Sirp-alpha-2|Signal-regulatory protein alpha-3|Sirp-alpha-3|p84|CD antigen CD172a

Accession Number:

NM_001040022.1

UniProt Number:

P78324

Gene Name:

SIRPA

Gene ID:

140885

Gene Aliases:

BIT|MFR|MYD1|PTPNS1|SHPS1|SIRP

Target Species:

Human

Cell Background:

Jurkat