PathHunter® HCRTR1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® HCRTR1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. orexin A) based internalization of HCRTR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Orexin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

OX1

Protein Aliases:

orexin receptor 1

Accession Number:

NM_001525

UniProt Number:

O43613

Gene Name:

HCRTR1

Gene ID:

3061

Gene Aliases:

OX1R

Target Species:

Human

Cell Background:

U2OS