PathHunter® Human GLP-1 (GLP-1 Receptor) Total GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human GLP-1 (GLP-1 Receptor) Total GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the GLP1R Total GPCR internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. exendin-4) based internalization of GLP1R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, the resulting internalization of the GLP1R receptor to the early endosome leads to production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Coupling:

Gs

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

U2OS