PathHunter® Human LPA3 (EDG7) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human LPA3 (EDG7) Activated GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the EDG7 Activated GPCR internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that affect GPCR activation and internalization. This cell line enables the assessment of ligand (e.g., LPA)- based internalization of EDG7 via the detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged EDG7. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting EDG7-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Lysophospholipid (LPA)

Sub Family:

Class A

Coupling:

Gq

Protein Name:

LPA3

Protein Aliases:

LPA-3|EDG7|Lysophosphatidic acid receptor Edg-7

Accession Number:

NM_012152.2

UniProt Number:

Q9UBY5

Gene Name:

LPAR3

Gene ID:

23566

Gene Aliases:

LP-A3|Edg-7|RP4-678I3|HOFNH30|LPA3

Target Species:

Human

Cell Background:

U2OS