PathHunter® CHO-K1 NPR1 Functional Assay

Background Information:

The PathHunter® CHO-K1 NPR1 Functional Assay is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting NPR1. This cell line enables assessment of ligand (e.g. ANP) based stimulation of receptor activity via detection of resulting increases in the levels of intracellular cGMP. This product uses EFC technology. The included cell line overexpresses untagged NPR1 Upon activation of the pathway, the increase in levels of intracellular cGMP leads to production of increased EFC signal when the cGMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Signaling Pathway

Family:

Guanylyl Cyclase

Sub Family:

Cyclic Nucleotide Signaling

Protein Name:

Atrial Natriuretic Factor (ANF)

Protein Aliases:

guanylate cyclase A

Accession Number:

NM_000906.3

UniProt Number:

P16066

Gene Name:

NPR1

Gene ID:

4881

Gene Aliases:

GUCY2A|ANPa

Target Species:

Human

Cell Background:

CHO-K1