PathHunter® CCR4 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® CCR4 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. CCL22/MDC) based internalization of CCR4 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged CCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR4 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR4

Protein Aliases:

CC-CKR-4|CCR-4|CCR4|K5-5|CD antigen CD194

Accession Number:

AY322539

UniProt Number:

P51679

Gene Name:

CCR4

Gene ID:

1233

Gene Aliases:

CC-CKR-4|CMKBR4|CKR4|k5-5|ChemR13|CD194

Target Species:

Human

Cell Background:

U2OS