KILR® Human PD-1 Stable Cell Line Cytotoxicity Assay (Jurkat)

Background Information:

The KILR Human PD-1 Stable Cell Line Cytotoxicity Assay (Jurkat) utilizes the KILR Jurkat PD-1 cell line. The cell line is a stable, engineered target line to measure direct cell death mediated by immuno-oncology treatments (e.g., checkpoint inhibitors, checkpoint agonists, bispecifics, CAR-T, and more) via Antibody-Dependent Cell-mediated Cytotoxicity (ADCC), Complement-Dependent Cytotoxicity (CDC), and Antibody-Dependent Phagocytosis (ADCP). This product uses EFC technology. The cell line overexpresses a ProLabel-tagged housekeeping gene as well as untagged PD-1. Once the cells have been lysed by effector cells, ProLabel is released into the media, where EA, contained in the detection kit, produces an EFC signal proportional to the level of ProLabel released. This allows for the calculation of the percent lysis of the cell line.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10 plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Inhibitory Receptor

Protein Name:

PD-1

Protein Aliases:

hPD-1|CD antigen CD279

Accession Number:

NM_005018.2

UniProt Number:

Q15116

Gene Name:

PDCD1

Gene ID:

5133

Gene Aliases:

PD1

Target Species:

Human

Cell Background:

Jurkat