InCELL Hunter™ A549 MEK1 Kinase Binding Cell Line

Background Information:

The InCELL Hunter™ A549 MEK1 Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of MEK1 via detection of MEK1 levels. This product uses EFC technology. The cell line included overexpresses MEK1 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

STE

Sub Family:

Protein Ser/Thr

Protein Name:

MEK1

Protein Aliases:

MAPKK 1|MKK1|ERK activator kinase 1|MAPK/ERK kinase 1|MEK 1

Accession Number:

AAB97145.1

UniProt Number:

Q02750

Gene Name:

MAP2K1

Gene ID:

5604

Gene Aliases:

MEK1|MAPKK1|PRKMK1

Target Species:

Human

Cell Background:

A549