cAMP Hunter™ CHO-K1 EDG1 Gi Cell Line

Background Information:

The cAMP Hunter™ EDG1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. sphingosine 1-phosphate) based activation of EDG1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type EDG1 , a naturally Gi/Go coupled GPCR. Upon activation of EDG1 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

S1P1

Protein Aliases:

EDG1|Endothelial differentiation G-protein coupled receptor 1|Sphingosine 1-phosphate receptor Edg-1|S1P receptor Edg-1|CD antigen CD363

Accession Number:

NM_001400

UniProt Number:

P21453

Gene Name:

S1PR1

Gene ID:

1901

Gene Aliases:

edg-1|D1S3362|CD363

Target Species:

Human

Cell Background:

CHO-K1