SPRINTer™ Human c-Myc Protein Turnover Biosensor Cell-Based Assay Kit (HCT-116)

Background Information:

The SPRINTer™ Human c-Myc Targeted Protein Degradation Cell-Based Assay Kit (HCT-116) utilizes the SPRINTer™ HCT-116 c-Myc Protein Turnover Biosensor Cell Line to monitor endogenous c-Myc protein levels and allow measurement of drug-induced targeted protein degradation (TPD). Genome engineering or editing techniques, such as CRISPR/Cas9, are used to introduce a small tag into the endogenous locus of interest. This allows for the quantitation of changes in c-Myc levels when cells are treated with therapeutic agents such as targeted protein degraders. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Nuclear Protein

Family:

Transcription factor

Sub Family:

Basic Leucine Zipper Domain

Protein Name:

MYC

Accession Number:

NM_002467.6

UniProt Number:

P01106

Gene Name:

MYC

Gene ID:

4609

Gene Aliases:

c-Myc|bHLHe39|MYCC

Target Species:

Human

Cell Background:

HCT-116