Ready-to-Assay MC2 Melanocortin Receptor Frozen Cells

Background Information:

The Ready-to-Assay MC2 Melanocortin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. adrenocorticotropin) based activation of MC2R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MC2R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MC2R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Protein Name:

MC2

Protein Aliases:

adrenocorticotropic hormone receptor

Accession Number:

NM_000529

UniProt Number:

Q01718

Gene Name:

MC2R

Gene ID:

4158

Gene Aliases:

ACTHR

Target Species:

Human

Cell Background:

Chem-1