Ready-to-Assay IP1 Prostanoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay IP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGIR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGIR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

IP

Protein Aliases:

PGI receptor|PGI2 receptor|Prostanoid IP receptor

Accession Number:

AY242134

UniProt Number:

P43119

Gene Name:

PTGIR

Gene ID:

5739

Gene Aliases:

IP|PRIPR

Target Species:

Human

Cell Background:

Chem-1