Ready-to-Assay EP4 Prostanoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay EP4 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGER4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER4 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP4

Protein Aliases:

PGE2 receptor EP4 subtype|Prostanoid EP4 receptor

Accession Number:

AY429109

UniProt Number:

P35408

Gene Name:

PTGER4

Gene ID:

5734

Gene Aliases:

EP4|PTGER2

Target Species:

Human

Cell Background:

HEK293