Ready-to-Assay EP2 Prostanoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay EP2 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGER2 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER2 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP2

Protein Aliases:

PGE2 receptor EP2 subtype|Prostanoid EP2 receptor

Accession Number:

NM_000956

UniProt Number:

P43116

Gene Name:

PTGER2

Gene ID:

5732

Gene Aliases:

EP2

Target Species:

Human

Cell Background:

HEK293