Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP1

Protein Aliases:

PGE2 receptor EP1 subtype|Prostanoid EP1 receptor

Accession Number:

NM_000955.2

UniProt Number:

P34995

Gene Name:

PTGER1

Gene ID:

5731

Gene Aliases:

EP1

Target Species:

Human

Cell Background:

HEK293