PathHunter® Human TrkB Functional Kinase Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human TrkB Functional Kinase Cell-Based Assay Kit (U2OS) utilizes the U2OS TrkB Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation via the detection of TrkB activation-induced SH2 domain protein recruitment. The included cell line overexpresses PK-tagged Human TrkB and an EA-tagged SH2 domain protein. Upon ligand (e.g. BDNF)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKB

Protein Aliases:

GP145-TrkB|Trk-B|Neurotrophic tyrosine kinase receptor type 2|TrkB tyrosine kinase|Tropomyosin-related kinase B

Accession Number:

NM_006180

UniProt Number:

Q16620

Gene Name:

NTRK2

Gene ID:

4915

Gene Aliases:

TRKB

Target Species:

Human

Cell Background:

U2OS