PathHunter® Human TIM3/TIM3 Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human TIM3/TIM3 Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS TIM3/TIM3 Dimerization Cell Line. This assay enables the assessment of anti-TIM3 antibody-induced dimerization of TIM3/TIM3. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing TIM3 fused to PK and another construct containing TIM3 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Checkpoint Receptor

Family:

T Cell Receptor

Protein Name:

TIM3

Protein Aliases:

T-cell immunoglobulin and mucin domain-containing protein 3|TIMD-3|T-cell immunoglobulin mucin receptor 3|TIM-3|T-cell membrane protein 3|CD antigen CD366

Accession Number:

NM_032782.4

UniProt Number:

Q8TDQ0

Gene Name:

HAVCR2

Gene ID:

84868

Gene Aliases:

TIM3|TIMD3

Target Species:

Human

Cell Background:

U2OS