PathHunter® Human PTGER1 β-Arrestin Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human PTGER1 β-Arrestin Cell-Based Assay Kit (U2OS) utilizes the Human PTGER1 β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., prostaglandin E2) based activation of PTGER1 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged PTGER1 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gq

Protein Name:

EP1

Protein Aliases:

PGE2 receptor EP1 subtype|Prostanoid EP1 receptor

Accession Number:

NM_000955.2

UniProt Number:

P34995

Gene Name:

PTGER1

Gene ID:

5731

Gene Aliases:

EP1

Target Species:

Human

Cell Background:

U2OS