PathHunter® Human PD-1/PD-1 Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human PD-1/PD-1 Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS PD-1/PD-1 Dimerization Cell Line. This assay enables the assessment of anti-PD1 antibody-induced dimerization of PD-1/PD-1. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing PD-1 fused to PK and another construct containing PD-1 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Checkpoint Receptor

Family:

T Cell Receptor

Protein Name:

PD-1

Protein Aliases:

hPD-1|CD antigen CD279

Accession Number:

NM_005018.2

UniProt Number:

Q15116

Gene Name:

PDCD1

Gene ID:

5133

Gene Aliases:

PD1

Target Species:

Human

Cell Background:

U2OS