PathHunter® Mouse NPY1R β-Arrestin Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Mouse NPY1R β-Arrestin Cell-Based Assay Kit (U2OS) utilizes the Mouse NPY1R β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., peptide YY) based activation of mouse NPY1R GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged mouse NPY1R target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Neuropeptide Y

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

Y1

Accession Number:

NM_010934.4

UniProt Number:

Q04573

Gene Name:

NPY1R

Gene ID:

18166

Target Species:

Mouse

Cell Background:

U2OS