Biological Information

Background Information:

The PathHunter® INSRb RTK Internalization Assay is a stable, engineered cell line for use in studying molecules targeting RTKs. This cell line enables assessment of RTK activation via detection of INSRb internalization to endosomes. This product uses EFC technology. The included cell line overexpresses PK-tagged INSRb and EA-tagged endosome fusion proteins. Upon ligand-induced activation of the receptor, internalization of the receptor to the endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

IR

Protein Aliases:

CD antigen CD220 [Cleaved into: Insulin receptor subunit alpha; Insulin receptor subunit beta]

Accession Number:

NM_000208

UniProt Number:

P06213

Gene Name:

INSR

Gene ID:

3643

Gene Aliases:

CD220

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Internalization

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

PathHunter®