PathHunter® Human IL-13 (IL4R/IL13RA1) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-13 (IL4R/IL13RA1) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IL4R/IL13RA1 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-13)-induced dimerization of IL4R/IL13RA1 pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IL4R fused to PK and IL13RA1 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

IL-2 Receptor

Protein Name:

IL4RA

Protein Aliases:

IL-4R subunit alpha|IL-4R-alpha|IL-4RA|CD antigen CD124 [Cleaved into: Soluble interleukin-4 receptor subunit alpha|Soluble IL-4 receptor subunit alpha|Soluble IL-4R-alpha|sIL4Ralpha/prot|IL-4-binding protein|IL4-BP]

Accession Number:

NM_000418.3

UniProt Number:

P24394

Gene Name:

IL4R

Gene ID:

3566

Gene Aliases:

IL4RA|582J2.1

Target Species:

Human

Cell Background:

U2OS