PathHunter® Human IL-17E (IL17RA/IL17RB) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-17E (IL17RA/IL17RB) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IL17RA/IL17RB Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-17E)-induced dimerization of IL17RA/IL17RB pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IL17RA fused to PK and IL17RB fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

IL-17 Receptor

Protein Name:

IL17RA

Protein Aliases:

IL-17RA|CDw217|CD antigen CD217

Accession Number:

NM_014339

UniProt Number:

Q96F46

Gene Name:

IL17RA

Gene ID:

23765

Gene Aliases:

IL17R

Target Species:

Human

Cell Background:

U2OS