PathHunter® Human IFN gamma (IFNGR1/IFNGR2) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IFN gamma (IFNGR1/IFNGR2) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IFNGR1/IFNGR2 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IFNγ)-induced dimerization of IFNGR1/IFNGR2 pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IFNGR1 fused to PK and IFNGR2 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

Interferon Receptor

Protein Name:

IFN-gamma R1

Protein Aliases:

IFN-gamma-R1|CDw119|Interferon gamma receptor alpha-chain|IFN-gamma-R-alpha|CD antigen CD119

Accession Number:

NM_000416.2

UniProt Number:

P15260

Gene Name:

IFNGR1

Gene ID:

3459

Target Species:

Human

Cell Background:

U2OS